MAB5520-I

Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 7.1

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 7.1, monoclonal
• mol peso: calculated mol wt 250.54 kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 100 μL
• técnicas: flow cytometry: suitable; immunoprecipitation (IP): suitable
• isotipo: IgG1
• secuencia del epítopo: Extracellular domain
• Nº de acceso Protein ID: NP_001888
• Nº de acceso UniProt: Q6UVK1
• Condiciones de envío: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... cspg4> CSPG4(1464)

Descripción

Descripción general

Chondroitin sulfate proteoglycan 4 (UniProt: Q6UVK1; also known as Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan) is encoded by the CSPG4 (also known as MCSP) gene (Gene ID: 1464) in human. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off to produce the mature form that contains a large extracellular domain (aa 30-2224), a transmembrane domain (aa 2225-2245), and a cytoplasmic domain (aa 2246-2322). NG2 plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration.

Especificidad

Clone 7.1 is a mouse monoclonal antibody that detects NG2 Chondroitin sulfate proteoglycan.

Inmunógeno

Human bone marrow stromal cells infected with SV-40.

Aplicación

Quality Control Testing

Evaluated by Flow Cytometry in A375 cells.

Flow Cytometry Analysis: 1 μg of this antibody detected NG2 Chondroitin Sulfate Proteoglycan in one million A375 cells.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated NG2 Chondroitin Sulfate Proteoglycan in Immunoprecipitation applications (Smith, F.O., et al. (1996). Blood;87(3):1123-33).

Flow Cytometry Analysis: A representative lot detected NG2 Chondroitin Sulfate Proteoglycan in Flow Cytometry applications (Morozov, A., et al. (2010). Clin Cancer Res.;16(23):5630-40; Behm, F.G., et al. (1996). Blood;87(3):1134-9; Smith, F.O., et al. (1996). Blood;87(3):1123-33).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-NG2 Chondroitin Sulfate Proteogycan, clone 7.1, Cat. No. MAB5520-I, is a mouse monoclonal antibody that detects NG2 Chondroitin sulfate proteoglycan and is tested for use in Flow Cytometry and Immunoprecipitation.

Forma física

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable