MAB13431

Anti-MMP-2 Antibody, pro and active form, clone A-Gel VC2

clone A-Gel VC2, Chemicon^®, from mouse

• Sinónimos: Gelatinase A, 72 kDa Type IV Collagenase
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: A-Gel VC2, monoclonal
• reactividad de especies: human
• fabricante / nombre comercial: Chemicon^®
• técnicas: ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• isotipo: IgG1
• idoneidad: not suitable for Western blot
• Nº de acceso NCBI: NM_004530.2
• Nº de acceso UniProt: P08253
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MMP2(4313)

Descripción

Descripción general

MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer or the stromal cells. There is a cooperation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction.

Especificidad

MAB13431 recognizes proteins of 72kDa and ~66kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72kDa collagenase IV or gelatinase A). CHEMICON MAB-13431 shows no cross-reaction with pro and active forms of other MMPs.

CELLULAR LOCALIZATION:

Cytoplasmic.

Inmunógeno

Epitope: pro and active form

Human APMA-activated native 72kDa Gelatinase A.

Aplicación

Anti-MMP-2 Antibody, pro & active form, clone A-Gel VC2 is an antibody against MMP-2 for use in ELISA, IH, IH(P).

Immunohistochemistry (Frozen & fixed-paraffin embedded) 2-4 μg/mL 30 min at RT. Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min.

ELISA (not suitable for coating, contains BSA) 1:200-1:2000

Not suitable for Western blotting


Optimal working dilution should be determined by the end user.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Ligadura / enlace

Replaces: 04-1048

Forma física

10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide

Format: Purified

Almacenamiento y estabilidad

Antibody is stable for 12 months when stored at 2-8ºC

Nota de análisis

Control
POSTIVE CONTROL:

Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable