DR1061

Anti-ENO1 Mouse mAb (8G8)

liquid, clone 8G8, Calbiochem^®

• Sinónimos: Anti-α Enolase 1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 8G8, monoclonal
• formulario: liquid
• no contiene: preservative
• reactividad de especies: human
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• isotipo: IgG1
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ENO1(2023)

Descripción

Descripción general

Anti-ENO1, mouse monoclonal, clone 8G8, recognizes the ~45-48 kDa ENO1 in MCF-7 and HeLa cells and in human lymphoma tissue. It is validated for use in ELISA, WB, ICC, & IHC (paraffin sections).

Purified mouse monoclonal antibody. Recognizes the ~45-48 kDa ENO1 protein.

Recognizes the ~45-48 kDa ENO1 protein in MCF-7 and HeLa cells and human lymphoma tissue.

Inmunógeno

Full-legth, recombinant, human, ENO1 (aa1-435), expressed as a GST fusion protein

Aplicación

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (1 g/ml)

Advertencia

Toxicity: Regulatory Review (Z)

Forma física

In PBS, pH 7.30.

Reconstitución

Following initial thaw, aliquot and freeze (-20°C).

Nota de análisis

Positive Control
MCF-7 cells, Human lymphoma tissue, HeLa cells

Otras notas

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Ho, J.A., et al. 2010. Anal. Chem.82 5944.
Zhao, J., et al. 2007. Mol. Cell Proteomics.6 987.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable