Saltar al contenido
Merck
Todas las fotos(2)

Key Documents

CC163-350UG

Sigma-Aldrich

ECMatrix-221 E8 Laminin Substrate

Xeno-free laminin-221 coating for the differentiation of pluripotent stem cells into cardiomyocytes, 350 μg (CHO-S derived)

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Código UNSPSC:
12352207
NACRES:
NA.81

formulario

liquid

envase

pkg of 2 X 175 μg

técnicas

cell culture | stem cell: suitable

temp. de almacenamiento

2-8°C

Descripción general

Cardiomyocytes are the cardiac muscle cells that make up the heart and are responsible for generating contractile forces within the heart. Laminin-221 is known to bind to the integrin α7X2β1 which is located on the cell surface of pluripotent stem cells and is known to induce cardiac and skeletal muscle differentaition. Professor Kiyotoshi Sekiguchi′s group (Matrixome, Inc.) have produced a recombinant E8 fragment of laminin-221 at large-scale while retaining the full integrin binding activity. ECMatrix-221 E8 Laminin Substrates are recombinant Laminin-221-E8 fragments which bind to integrin α7X2β1 and increases the differentiation of pluripotent stem cells (ES/iPSCs) into functional cardiomyocytes.

Aplicación

Xeno-free laminin-221 coating for the differentiation of pluripotent stem cells into cardiomyocytes, 350 μg (CHO-S derived)

Envase

350 μg (2 x175 μg)

Componentes

2 X 175 μg ECMatrix-221 E8 Laminin Substrate (0.5 mg/mL in PBS). Expressed in CHO-S cells.

Calidad

  • Purity (SDS-Page): > 95%
  • Endotoxin Test: = 750 EU/mg
  • Mycoplasma Test: Negative
  • Sterility Test: Negative
  • Integrin Binding Assay (kDa): = 10 nM

Nota de preparación

Precoating Method
1. Dilute the 0.5 mg/mL stock solution with sterile PBS to achieve a 2.5 μg/mL working solution.
2. Coat dishes with ECMatrix-221 at 0.25 μg/cm2 (for example, for one well of a 6-well plate add 1 mL of the 2.5 μg/mL working solution).
3. Incubate for 1 hour at 37°C, 3 hours at room temperate or overnight at 4°C.
4. Before use, remove remaining fluid from the coated surface (do not rinse).
5. Detach cells into small clumps using Accutase.
6. Plate the cells at desired density.

Note: Do not allow the plates to dry, briefly spin down all liquids in the tube before use, avoid repeated freeze-thaw cycles.

Almacenamiento y estabilidad

ECMatrix-221 E8 Laminin Substrates should be stored at 2-8°C. Avoid multiple freeze-thaw cycles and protect from light.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico