AP202A

Goat Anti-Rat light chain Antibody, Alkaline Phosphatase conjugate

Chemicon^®, from goat

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: goat
• conjugado: alkaline phosphatase conjugate
• forma del anticuerpo: affinity purified immunoglobulin
• tipo de anticuerpo: secondary antibodies
• clon: polyclonal
• reactividad de especies: rat
• fabricante / nombre comercial: Chemicon^®
• técnicas: ELISA: suitable; western blot: suitable
• isotipo: IgG
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Light chains covalently bound to a pair of heavy chains to form an antibody molecule. Immunoglobulin light chains occur in two types, kappa or lambda. Light chain′s mass is about 25,000 Daltons per mole. Each light chain consists of about 250 amino-acids long. These are usually designated by the Greek letters kappa and lambda. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Especificidad

Minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep.

The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep immunoglobulins.

Inmunógeno

Epitope: Kappa light chain

Prepared from purified rat IgG light chain.

Aplicación

Detect light chain using this Goat anti-Rat light chain Antibody, Alkaline Phosphatase conjugate validated for use in ELISA & WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

Western Blotting: 1:5,000 -1:50,000

ELISA: 1:5,000 -1:50,000

Optimal working dilutions must be determined by the end user.

Descripción de destino

25 kDa

Forma física

ImmunoAffinity Purified

Purified by immunoaffinity chromatography. Liquid in 0.01M Tris-HCl, 0.25M NaCl, pH 8.0, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer.

Almacenamiento y estabilidad

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3