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ABT241

Sigma-Aldrich

Anti-acetyl Tubulin (Lys40) Antibody

from rabbit, purified by affinity chromatography

Sinónimos:

Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

human

reactividad de especies (predicha por homología)

rat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)

técnicas

western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

acetylation (Lys40)

Información sobre el gen

human ... TUBA1A(7846)

Descripción general

Tubulin is the major constituent of microtubules, and microtubules are major cytoskeletal elements. Tubulin proteins undergo a number of post-translational modifications both during individual synthesis and after complete tubule formation. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects the affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

Inmunógeno

Epitope: Near N-Terminus
KLH-conjugated linear peptide corresponding to a region near the near N-Terminus of human Tubulin acetylated at Lys40.

Aplicación

Anti-acetyl Tubulin (Lys40) Antibody is an antibody against acetyl Tubulin (Lys40) for use in western blotting.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Calidad

Evaluated by Western Blotting in untreated and Trichostatin A treated HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl Tubulin (Lys40) in 10 µg of Trichostatin A treated HeLa cell lysate, and not in untreated HeLa cell lysate.

Descripción de destino

~50 kDa observed

Forma física

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Kylie Jacobs et al.
mBio, 11(1) (2020-02-13)
Toxoplasma gondii's single mitochondrion is very dynamic and undergoes morphological changes throughout the parasite's life cycle. During parasite division, the mitochondrion elongates, enters the daughter cells just prior to cytokinesis, and undergoes fission. Extensive morphological changes also occur as the
Joseph M Varberg et al.
mSphere, 1(1) (2016-06-16)
Toxoplasma gondii is a widespread protozoan parasite that causes potentially life-threatening opportunistic disease. New inhibitors of parasite replication are urgently needed, as the current antifolate treatment is also toxic to patients. Microtubules are essential cytoskeletal components that have been selectively
Rodolpho Ornitz Oliveira Souza et al.
Journal of cell science, 135(22) (2022-11-01)
The single mitochondrion of Toxoplasma gondii is highly dynamic, being predominantly in a peripherally distributed lasso-shape in intracellular parasites and collapsed in extracellular parasites. The peripheral positioning of the mitochondrion is associated with apparent contacts between the mitochondrion membrane and
Ayad A Al-Hamashi et al.
Bioorganic chemistry, 116, 105297-105297 (2021-09-13)
Despite the advances in treatment strategies, cancer is still the second leading cause of death in the USA. A majority of the currently used cancer drugs have limitations in their clinical use due to poor selectivity, toxic side effects and
Rayna Rosati et al.
Molecular pharmacology, 90(3), 225-237 (2016-07-07)
Histone deacetylase inhibitors (HDACIs) can disrupt the viability of prostate cancer (PCa) cells through modulation of the cytosolic androgen receptor (AR) chaperone protein heat shock protein 90 (HSP90). However, toxicities associated with their pleiotropic effects could contribute to the ineffectiveness

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