ABT1485

Anti-Actin

from rabbit, purified by affinity chromatography

• Sinónimos: Actin, alpha skeletal muscle, Alpha-actin-1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: mouse, human
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Nº de acceso NCBI: NP_001091
• Nº de acceso UniProt: P68133
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ACTA1(58)

Descripción

Descripción general

Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3). Although actins show >90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic - and -actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins.

Especificidad

Broad species reactivity expected due to 100% sequence homology, including Bovine, Horse, Non-Human Primate, Porcine, Rabbit, and Rat.

This rabbit polyclonal antibody was raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).

Inmunógeno

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).

Aplicación

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in A431 and NIH/3T3 cells.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected actin in human cardiac muscle, skeletal muscle, and small intestine tissue sections.

This polyclonal antibody is also available as Alexa Fluor^™ 488 and Alexa Fluor 647 conjugates (Cat. No. ABT1485-AF488 and ABT1485-AF647) for flow cytometry, immunocytochemistry, immunofluorescence applications.

Research Category
Cell Structure

This rabbit polyclonal Anti-Actin Antibody, Cat. No. ABT1485 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Actin.

Calidad

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected actin in 10 µg of human skeletal muscle tissue lysate.

Descripción de destino

~42.0 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Información legal

ALEXA FLUOR is a trademark of Life Technologies

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable