ABT146

Anti-Occludin Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Occludin
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology), canine (based on 100% sequence homology), primate (based on 100% sequence homology), rat (based on 100% sequence homology), equine (based on 100% sequence homology)
• envase: antibody small pack of 25 μg
• técnicas: western blot: suitable
• Nº de acceso NCBI: NP_002529
• Nº de acceso UniProt: Q16625
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... OCLN(4950) , OCLN(100506658)

Descripción

Descripción general

Occludin is a 65-kDa integral plasma-membrane protein located at the tight junctions. It is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Occludin is believed to prevent the transport of macromolecules through the tight junction. Encoded by the OLCN gene, occludin spans the plasma membrane four times, forming two extracellular loops and exposing its NH2 and COOH terminus to the cytosol. This cytoplasmic tail binds directly to the ZO family of proteins, which link the protein complex to the actin cytoskeleton. Phosphorylation/dephosphorylation plays a major role in regulation of occludin and tight junctions. Expression of occludin is required for infection of liver cells by hepatitis C virus (HCV).

Especificidad

This antibody recognizes the N-terminus of Occludin.

Inmunógeno

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human Occludin.

Aplicación

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Occludin Antibody is validated for use in Western Blotting for the detection of Occludin.

Calidad

Evaluated by Western Blot in human kidney tissue lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected Occludin in 10 µg of human kidney tissue lysate.

Descripción de destino

~63 kDa observed. The calculated molecular weight is 63 kDa, however Occludin has been shown ranging from ~55 to ~65 kDa in western blots (Florian, P., et al. (2003). Biochemical and Biophysical Research Communications. 304:5-10).

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Human kidney tissue lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.