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Merck
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ABS787

Sigma-Aldrich

Anti-PGK1 Antibody (Center)

from rabbit

Sinónimos:

PGK1, PGKA, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

reactividad de especies

human, mouse

técnicas

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Nº de acceso UniProt

Condiciones de envío

dry ice

Información sobre el gen

human ... PGK1(5230)

Descripción general

Phosphoglycerate kinase 1 (PGK1) and encoded by the gene names Pgk1 or Pgk-1, is important for phosphoprotein glycolysis. PGK1 is an ATP-generating glycolytic enzyme that forms part of the glycolytic pathway that is often highly expressed in prostate cancer and is regulated by hypoxia-inducible factor-1α. Extracellular PGK1 facilitates the cleavage of plasminogen generating the vascular inhibitor angiostatin.

Inmunógeno

Epitope: Central region
KLH-conjugated synthetic peptide corresponding to the central region of human PGK1.

Aplicación

Detect PGK1 using this rabbit polyclonal Anti-PGK1 Antibody, Cat. No. ABS787, validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, and Flow Cytometry.
Research Category
Metabolism
Research Sub Category
Glucose/Glycogen Metabolism
Western Blotting Analysis (WB): A 1:1000 dilution from a representative lot of this antibody detected PGK1 in multiple lysates. See chart for reactivity detail.

Immunohistochemistry Analysis (IHC): A 1:10-50 dilution from a representative lot detected PGK1 in human cancer tissue.

Immunocytochemistry Analysis (ICC): A 1:10-50 dilution from a representative lot detected PGK1 in HeLa cells.

Flow Cytometry (FC): A 1:10-50 dilution of this antibody detected PGK1 in HeLa cells.

Calidad

Evaluated by Western Blotting in HT1376 cell lysate.

Western Blotting Analysis (WB): A 1:1000 dilution of this antibody detected PGK1 in HT1376 cell lysate.

Descripción de destino

~48 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Forma física

Ammonium Sulfate
Format: Purified
Purified rabbit polyclonal in buffer containing PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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