ABN1018

Anti-LRP1 Antibody, Intracellular domain

serum, from rabbit

• Sinónimos: Prolow-density lipoprotein receptor-related protein 1, A2MR, Alpha-2-macroglobulin receptor, APOER, Apolipoprotein E receptor, CD91, LRP-1, LRP-85, LRPICD
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: mouse
• reactividad de especies (predicha por homología): human (based on 100% sequence homology), rat (based on 100% sequence homology)
• técnicas: western blot: suitable
• Nº de acceso NCBI: NP_002323
• Nº de acceso UniProt: Q07954
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... LRP1(4035)

Descripción

Descripción general

Prolow-density lipoprotein receptor-related protein 1 (UniProt Q07954; also known as A2MR, Alpha-2-macroglobulin receptor, APOER, Apolipoprotein E receptor, CD91, LRP-1) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID 4035) in human. LRP1 is an LDLR family member expressed by vascular endothelial cells forming the blood-brain barrier (BBB), vascular mural cells (pericytes and vascular smooth muscle cells (VSMCs)), neurons, and astrocytes. LRP1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (apoE), APP and Aβ. LRP1 is the primary receptor mediating transport of Aβ across the BBB into circulation, thereby clearing it from the brain. LRP1 is synthesized with a signal peptide sequence (a.a. 1-19), posttranslational cleavage yields a soluble form (sLRP1) and a cell surface-bound form composed of an 515 kDa extracellular α-chain (LRP-515) and a 85 kDa single-transmembrane (a.a. 4420-4444) β-chain (LRP-85) that contains the 100-amino intracellular domain (LRPICD; a.a. 4445-4544). BACE-1, ADAM-10, ADAM-12, ADAM-17, MT1-MMP, and t-PA are shown to be involved in the shedding of LRP1 to produce sLRP1.

Especificidad

Recognizes the Pro- and mature LRP-1 (a.a. 1-4544 and 20-4544, respectively), the beta-secretase-cleaved, membrane-bound LRP-85, and the gamma-secretase-cleaved intracellular domain LRPICD fragment, but not the beta-secretase-cleaved extracellular LRP-515 fragment.

Inmunógeno

Epitope: LRPICD.

GST-tagged recombinant human LRP1 intracellular domain (LRPICD).

Aplicación

Anti-LRP1 Antibody, Intracellular domain is an antibody against LRP1 for use in Western Blotting.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Western Blotting Analysis: A representative lot detected the LRP-85 target band in NIH/3T3 cell lysate, as well as in mouse brain and liver homogenates (Courtesy of Professor Nicola Zambrano, University of Naples Federico II, Italy).
Western Blotting Analysis: A representative lot detected a decreased LRP-85 target band in lysates from LRP1 shRNA-treated NIH/3T3 cells (Courtesy of Professor Nicola Zambrano, University of Naples Federico II, Italy).

Calidad

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected the LRP-85 target band in 10 µg of NIH/3T3 cell lysate.ysate.

Descripción de destino

~80 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forma física

Rabbit polyclonal antibody serum with 0.05% sodium azide.

Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable