ABE198

Anti-Sirt5 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: NAD-dependent deacetylase sirtuin-5, SIR2-like protein 5
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 4

Propiedades

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human
• species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_036373
• UniProt accession no.: Q9NXA8
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SIRT5(23408)

Descripción

General description

Sirtuin (SIRT5) is known as a nicotinamide adenine dinucleotide- dependent deacetylase that is a member of the silent information regulator 2 (Sir2) family of proteins that is important in aging, metabolism and disease. SIRT5 is expressed in a variety of tissues. Two different isoform have been described. Isoform 1 is reported to be express in the mitochondria and cytoplasm while isoform 2 appears to be localized to the mitochondria only. SIRT5 has also been linked to many primate-specific functions. SIRT5 has recently been implicated as having a role in adaptation to fasting, caloric restriction and high protein diets.

~34 kDa observed. Two isoforms at ~33 and ~34 kDa are known to exist.

Immunogen

KLH-conjugated linear peptide corresponding to human Sirt5.

Application

Immunohistochemistry Analysis: 1:500 dilution of a representative lot of this antibody detected Sirt5 in convoluted tubules of normal rat kidney tissue.

Use Anti-Sirt5 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Sirt5 also known as NAD-dependent deacetylase sirtuin-5, SIR2-like protein 5.

Analysis Note

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Sirt5 on 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable