ABE1959

Anti-RAD9A Antibody

from rabbit

• Sinónimos: Cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A, hRAD9, mRAD9, Rad9-like protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: human, mouse
• técnicas: immunohistochemistry: suitable (paraffin)
• Nº de acceso NCBI: NP_035367
• Nº de acceso UniProt: Q9Z0F6
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... RAD9A(5883) ; mouse ... Rad9A(19367)

Descripción

Descripción general

Cell cycle checkpoint control protein RAD9A (EC 3.1.11.2; UniProt Q9Z0F6; also known as DNA repair exonuclease rad9 homolog A, mRAD9, Rad9-like protein) is encoded by the Rad9a (also known as Rad9) gene (Gene ID 19367) in murine species. RAD9A is a component of the trimeric PCNA-like 9-1-1 complex (Rad9A, Rad1, and Hus1) and plays an important role in maintaining genomic integrity. RAD9A participates in both ATR and ATM signaling pathways-mediated cell cycle checkpoints in mitosis. Evidences indicate that RAD9A participates in DNA damage repair by interacting with proteins involved in base excision repair, mismatch repair and homologous recombination repair. RAD9A is shown to colocalize with γH2AX following DNA damage induction in an ATM-independent manner, and RAD9A inactivation delays the appearance of ionizing radiation-induced γH2AX foci. Rad9a-knockout causes embryonic lethality in mice due to increased apoptotic activity and reduced cellular proliferation, while mice heterozygous for Rad9a are prone to spontaneous and radiation-induced cataractogenesis. In addition, conditional knockout in keratinocytes causes enhanced susceptibility to carcinogen-induced skin tumors, while conditional Rad9a deletion in undifferentiated spermatogonia results in drastic effects on meiosis.

Especificidad

This rabbit polyclonal antibody detected nuclear RAD9A immunoreactivity in human and mouse tissue sections.

Inmunógeno

GST-tagged recombinant full-length mouse RAD9A.

Aplicación

Anti-RAD9A Antibody, Cat. No. ABE1959, is a highly specific rabbit polyclonal antibody that targets RAD9A and has been tested in Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50 dilution of from a representative lot detected RAD9A in mouse spleen tissue section.

Immunohistochemistry Analysis: A representative lot detected strong RAD9A immunoreactivity in the nuclei of prophase spermatocytes, lower immunoreactivity was also seen in Sertoli cells, while little or no staining of spermatids and spermatogonia was seen in 3.7% paraformaldehyde-fixed, paraffin-embedded mouse testis tissue sections (Vasileva, A., et al. (2013). J. Cell Sci. 126(Pt 17):3927-3938).

Research Category
Epigenetics & Nuclear Function

Calidad

Evaluated by Immunohistochemistry in human prostate cancer tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected RAD9A in human prostate cancer tissue section.

Descripción de destino

42.55/42.06 kDa (human/mouse) calculated.

Forma física

Format: Purified

Protein A purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt..

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable