ABE1414

Anti-MEIOB Antibody

serum, from rabbit

• Sinónimos: Meiosis-specific with OB domain-containing protein, Protein expressed in male leptotene and zygotene spermatocytes 675, MLZ-675
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: mouse
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_083473
• Nº de acceso UniProt: Q9D513
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Meiob(75178)

Descripción

Descripción general

MEIOB is a single stranded binding protein required for proper homologous recombination in meiosis I. MEIOB is required for proper DNA strand repair at double strand breaks and faithful synapsis of the chromosomes. MEIOB is also required for successful completion of meiotic recombination and is part of a multiprotein complex with RPA2 and SPATA22 proteins. MEIOB is expressed in the gonads and specifically in ovary and testis starting at the 14th weeks post fertilization. MEIOB is also expressed in a variety of tumors but not in other tissues other than testis and ovary. Because of its unique expression MEIOB is being examined as a possible anti-cancer autoimmune agent.

Inmunógeno

Recombinant protein corresponding to mouse MEIOB.

Aplicación

Immunoprecipitation Analysis: A representative lot immunoprecipitated MEIOB in P18 testes (Luo, M., et al. (2013). Nature. DOI: 10.1038/ncomms3788 pg. 1-12).
Immunocytochemistry Analysis: A representative lot detected MEIOB in mouse spermatocytes (Luo, M., et al. (2013). Nature. DOI: 10.1038/ncomms3788 pg. 1-12).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-MEIOB Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of MEIOB.

Calidad

Evaluated by Western Blotting in mouse testis tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MEIOB in 10 µg of mouse testis tissue lysate.

Descripción de destino

~50 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forma física

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable