ABC424

Anti-Sec23A Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Protein transport protein Sec23A, SEC23-related protein A
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: rat, mouse
• reactividad de especies (predicha por homología): human (based on 100% sequence homology)
• técnicas: western blot: suitable
• Nº de acceso NCBI: NP_033173
• Nº de acceso UniProt: Q01405
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SEC23A(10484) ; mouse ... Sec23A(20334) ; rat ... Sec23A(58817)

Descripción

Descripción general

The protein named Sec23a, also known as Sec23r, and encoded by the gene Sec23a/Sec23/Sec23r is a component of the COPII coat complex; this complex is the complex that governs ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. The transport of proteins in eukaryotic cells from the endoplasmic reticulum (ER) to the Golgi complex proceeds by deformation of specialized portions of the donor membrane to form carrier vesicles. A group of cytosolic proteins collectively known as COPII carry out a programmed set of sequential interactions, leading to cargo sorting and vesicle budding. The Sec23A protein is critical to proper protein transport in and out of cells, and it is localized to the smooth endoplasmic reticulum membrane where it is a peripheral membrane protein and interacts with the Golgi apparatus membranes as well. Sec23A is expressed at high levels in brain and fibroblasts as well as liver and other metabolically active tissues. Sec23A interacts with SEC23IP, HTR4, SEC16A and SLC6A proteins. Sec23A also appears to play a role directly in development as recent research linked Sec23A promoters to important developmental transcription factors such as TFII-1 and showed that important craniofacial and dental developmental genes could contribute to the WBS-specific facial dysmorphism in Williams-Beuren syndrome.

Inmunógeno

Linear peptide corresponding to mouse Sec23A.

Aplicación

Detect Sec23a using this rabbit polyclonal antibody, Anti-Sec23A Antibody validated for use in western blotting.

Western Blotting Analysis: 1 µg/mL from a representative lot detected Sec23A in 10 µg of mouse heart, thymus, pancreas, testis, and adrenal gland tissues.

Western Blotting Analysis: A representative lot from an independent laboratory detected Sec23A in various mouse tissues expressing wildtype Sec23A (Tao, J., et al. (2012). Proc Natl Acad Sci USA. 109(29):E2001-2009.).

Calidad

Evaluated by mouse brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Sec23A in 10 µg of mouse brain tissue lysate.

Descripción de destino

~86 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable