ABC137

Anti-ATPIF1 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: ATPase inhibitor, mitochondrial, Inhibitor of F(1)F(o)-ATPase, IF(1), IF1, ATPIF1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human, mouse
• técnicas: immunocytochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_057395
• Nº de acceso UniProt: Q9UII2
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ATPIF1(93974)

Descripción

Descripción general

ATPIF1 is an interesting protein that acts on ATP synthase and prevents it from catabolizing ATP when the mitochondrial membrane potential falls below a threshold, which ATP synthase starts to do by hydrolyzing ATP to pump protons out of the mitochondrial matrix to restore ion balance. ATPIF1 also indirectly regulates heme synthesis by modulating the mitochondrial pH and redox potential. ATPIF1 is expressed in the mitochondria and its active is determined by conformation. Homodimers are active, homotetramers are inactive and their formation is governed by pH. ATPIF1, because of its effects on ATP and heme, is important in cellular development, heme biosynthesis, energy metabolism and angiogenesis and erythrocytes differentiation.

Inmunógeno

Epitope: N-terminal inhibitory region & Antiparallel alpha-helical coiled region

GST-tagged recombinant protein corresponding to the N-terminal inhibitory region & Antiparallel alpha-helical coiled eegion of human ATPIF1.

Aplicación

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-ATPIF1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of ATPIF1.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected ATPIF1 in 10 µg of HEK293 cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected ATPIF1 in HeLa, A431, and NIH/3T3 cells.

Calidad

Evaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected ATPIF1 in 10 µg of T47D cell lysate.

Descripción de destino

~12 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable