AB9730

Anti-14-3-3 β Antibody

serum, Chemicon^®

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: rabbit, bovine, human, rat, mouse, sheep
• fabricante / nombre comercial: Chemicon^®
• técnicas: ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NM_003404.3; NM_139323.2
• Nº de acceso UniProt: P31946
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... YWHAB(7529)

Descripción

Descripción general

Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer′s.

The 14-3-3 beta antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer′s and Parkinson′s Disease.

Especificidad

14-3-3 beta. By Western blot the antibody recognizes the ~30 kDa protein.

Inmunógeno

Synthetic peptide from the N-terminal area of sheep 14-3-3 beta.

Aplicación

Detect 14-3-3 β using this Anti-14-3-3 β Antibody validated for use in ELISA, WB, IH.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Western blot: 1:3,000 using ECL
Immunohistochemistry: 1:300-1:600 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes.
ELISA

Optimal working dilutions must be determined by end user.

Descripción de destino

30 kDa

Ligadura / enlace

Replaces: 04-1089

Forma física

Liquid.

Serum

Almacenamiento y estabilidad

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable