AB5898

Anti-Protein Gene Product 9.5 Antibody

serum, Chemicon^®

• Sinónimos: PGP 9.5, UCH-L1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC
• Citations: 24

Propiedades

• biological source: guinea pig
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, rat, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable
• NCBI accession no.: NM_004181.3
• UniProt accession no.: P09936
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... UCHL1(7345) ; mouse ... Uchl1(22223) ; rat ... Uchl1(29545)

Descripción

Immunogen

A 17 amino acid peptide corresponding to amino acids 175-191 from soluble cytoplasmic human PGP9.5.

Application

Anti-Protein Gene Product 9.5 Antibody detects level of Protein Gene Product 9.5 & has been published & validated for use in IH.

Immunohistochemistry: 1:500.

Optimal working dilutions must be determined by end user.

blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5898 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Biochem/physiol Actions

Recognizes Protein Gene Product 9.5 (PGP9.5).

Physical form

Liquid. Contains 0.05% sodium azide.

Unpurified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• wgk: WGK 1