AB3468
Anti-Heat Shock Protein 90β Antibody
Chemicon®, from rabbit
Sinónimos:
HSP84
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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IHC, IP, WB
Citations:
9
Servicio técnico
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Permítanos ayudarlebiological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HSP90AA1(3320)
mouse ... Hsp90Aa1(15519)
rat ... Hsp90Aa1(299331)
Immunogen
Synthetic peptide corresponding to amino acids 2-13 from the N-terminus of mouse heat shock protein 90b.
Application
Immunoblotting: 5 μg/mL
Immunohistochemistry (frozen and formalin/paraffin): 5-10 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunocytochemistry
Immunoprecpitation: 10 μg/mg of protein lysate.
Optimal working dilutions must be determined by end user.
Immunohistochemistry (frozen and formalin/paraffin): 5-10 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunocytochemistry
Immunoprecpitation: 10 μg/mg of protein lysate.
Optimal working dilutions must be determined by end user.
Research Category
Protein Trafficking
Protein Trafficking
Research Sub Category
Chaperones
Chaperones
This Anti-Heat Shock Protein 90β Antibody is validated for use in IP, WB, IC, IH(P) for the detection of Heat Shock Protein 90β.
Biochem/physiol Actions
Reacts with a protein of 84 kD identified as heat shock protein 90beta (HSP90beta). No reactivity with HSP90alpha/HSP86.
CELLULAR LOCALIZATION: Nuclear and Cytoplasmic.
CELLULAR LOCALIZATION: Nuclear and Cytoplasmic.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Analysis Note
Control
POSITIVE CONTROL: MAD109 cells or breast carcinoma.
POSITIVE CONTROL: MAD109 cells or breast carcinoma.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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