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AB3202

Anti-LIM-3 Antibody

Chemicon®, from rabbit

Sinónimos:

Anti-HMFN1661

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IHC (p), IP, WB
Citations:
7


biological source

rabbit

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

fish, frog, mouse, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ABLIM3(22885)

Immunogen

C-terminal portion of mouse LIM-3 protein.

Application

Anti-LIM-3 Antibody is an antibody against LIM-3 for use in IP, WB, IC, IH, IH(P).
Immunohistochemistry: 1:250-1:1,000 on paraffin embedded sections. 1:3,000-1:6,000 on frozen sections. 1:4,000-1:8,000 on whole mounts. Recommended fixative MEMFA.

Immunocytochemistry: 1:200-1:500

Western blot: 1:2,500-1:5,000

Immunoprecipitation: 1:200

This antibody will work well in immunofluorescence. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Neuronal & Glial Markers

Biochem/physiol Actions

Recognizes LIM-3. This antibody does not appear to cross react with gsh-4 on tissue sections but may cross react in Western blot and immunoprecipitation.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Este artículo
SAB1407158AB3200ZRB1098
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit (recombinant)

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

Gene Information

human ... ABLIM3(22885)

Gene Information

human ... ABLIM3(22885)

Gene Information

human ... LHX1(3975)

Gene Information

human ... POU4F1(5457)

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

western blot: 1 μg/mL

technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable (peptide)

UniProt accession no.

O94929

UniProt accession no.

O94929

UniProt accession no.

P48742

UniProt accession no.

Q01851


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