AB15500

Anti-Calcium Transporter 1 Antibody

Chemicon^®, from rabbit

• Sinónimos: CaT-1, ECAC2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 300
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: rat
• fabricante / nombre comercial: Chemicon^®
• técnicas: ELISA: suitable; western blot: suitable
• Nº de acceso NCBI: NM_018646.2
• Nº de acceso UniProt: Q9H1D0
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: rat ... Slc7A1(25648)

Descripción

Descripción general

CaT-1 (rat/mouse 727 aa; human 725 aa, chromosome 7q33-q34; ~85 kDa) is also known as ECAC2, CAT-like (CATL) or TRPV6 (transient receptor potential cation channel, subfamily V, member 6) contains a cytoplasmic N-terminus, 6 ankyrin repeat domains, 6 transmembrane domains, an extracellular pore region between TM5-6, and cytoplasmic C-terminus. CaT-1 shows 75% sequence identity to epithelial Ca++ channel (ECaC) protein from rabbit kidney. In rat, CaT-1 transcript (3-Kb) is expressed in duodenum, proximal jejunum cecum and colon but not in kidney; whereas ECaC in rabbit is expressed in apical cell membranes in distal part of nephron (kidney), duodenum, jejunum and placenta.

Especificidad

Calcium transporter 1 (CaT-1, ECAC2). The immunogen sequence has no significant homology with CaT-2 or other proteins.

Other species have not yet been tested. The immunogen sequence is 100% conserved in mouse, 88% in humans and 94% in Cat-1/htrp8A and htrp8b proteins.

Inmunógeno

Synthetic peptide near the C-terminus of rat CaT-1.

Aplicación

Anti-Calcium Transporter 1 Antibody detects level of Calcium Transporter 1 & has been published & validated for use in ELISA & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1-10 µg/mL using ECL. The expected size of CaT-1 is ~83 kDa. However, posttranslational modification may alter its size depending upon the species and cell type.
Immunohistochemistry: Not tested. It is recommended that the antibody be tried at 2-20 µg/mL
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide/well.
Optimal working dilutions must be determined by end user.

Forma física

ImmunoAffinity Purified

Liquid in PBS with 0.1% BSA.

Almacenamiento y estabilidad

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids