AB135
Anti-Tryptophan Antibody
serum, Chemicon®
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eCl@ss:
32160702
UNSPSC Code:
12352203
Servicio técnico
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Permítanos ayudarlebiological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
shipped in
dry ice
target post-translational modification
unmodified
Immunogen
Tryptophan-gluteraldehyde-Poly-lysine
Application
Anti-Tryptophan Antibody is an antibody against Tryptophan for use in IH.
Immunohistochemistry: 1:1,000-1:2,500 by PAP (see suggested protocol).
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Biochem/physiol Actions
Tryptophan
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
Tryptophan-G-PL 1
5-Hydroxytryptophan-G-PL .01
5-Methoxytryptophan-G-PL .004
Tryptophan=G=PL (non reduced) .002
Tryptophan .0003
Tryptamine-G-PL .00009
5-Methoxytryptamine-G-PL .00002
5-Hydroxytryptamine-G-PL .000004
The antisera was also tested for specificity using the free-floating PAP technique on rat brain.
Abbreviations:
(G)Glutaraldehyde
(=)Non-reduced conjugate
(PL)Poly-lysine
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
Tryptophan-G-PL 1
5-Hydroxytryptophan-G-PL .01
5-Methoxytryptophan-G-PL .004
Tryptophan=G=PL (non reduced) .002
Tryptophan .0003
Tryptamine-G-PL .00009
5-Methoxytryptamine-G-PL .00002
5-Hydroxytryptamine-G-PL .000004
The antisera was also tested for specificity using the free-floating PAP technique on rat brain.
Abbreviations:
(G)Glutaraldehyde
(=)Non-reduced conjugate
(PL)Poly-lysine
Physical form
Rabbit antiserum. Liquid with 0.05% sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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