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70622-M

Sigma-Aldrich

Tuner Competent Cells - Novagen

Escherichia coli, rod shaped

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About This Item

Código UNSPSC:
41106202

product name

Tuner Competent Cells - Novagen, Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

origen biológico

Escherichia coli

Nivel de calidad

100

fabricante / nombre comercial

Novagen®

condiciones de almacenamiento

OK to freeze
avoid repeated freeze/thaw cycles

modo de crecimiento

adherent or suspension

morfología

rod shaped

técnicas

microbiological culture: suitable

transformación celular

transformation efficiency: >2×106 cfu/μg

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Descripción general

Genotype: F–ompT hsdSB (rB– mB–) gal dcm lacY1





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Tuner strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins.
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.

Componentes

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlTuner Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

Advertencia

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Información legal

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Kalle Sipilä et al.
Developmental cell, 57(12), 1453-1465 (2022-06-08)
Stem cell renewal and differentiation are regulated by interactions with the niche. Although multiple cell populations have been identified in distinct anatomical compartments, little is known about niche-specific molecular factors. Using skin as a model system and combining single-cell RNA-seq

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