539481-M

PKA, Catalytic Subunit, Mouse, Recombinant, E. coli

• Sinónimos: cAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
• Comisión internacional de enzimas: 2.7.1.37 (BRENDA, IUBMB)
• Código UNSPSC: 12352202

Propiedades

• Formulario: liquid
• Nivel de calidad: 100
• actividad específica: ≥2.5 x 10⁶ U/mL
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• actividad extraña: Phosphatases, proteases, none detected
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Recombinant, mouse PKA catalytic subunit expressed in E. coli. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca²⁺ homeostasis, and ion channel function. Suitable for studying the phosphorylation of purified substrates and for use with cell extracts. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.

Recombinant, mouse PKA catalytic subunit expressed in E. coli. The catalytic subunit of protein kinase A that combines with the regulatory subunit, in the absence of cAMP, to form the inactive PKA holoenzyme. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation. PKA-Cα is reported to potentiate IP₃-induced Ca²⁺ mobilization in isolated hepatocytes. Suitable for studies of PKA phosphorylation in vitro with purified substrates or following the introduction into intact cells or cell extracts.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; Cat. No. 05-23-4900) per min at 30°C, pH 7.5.

Forma física

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.

Reconstitución

Following initial thaw, aliquot and freeze (-20°C).

Otras notas

Grant, B.D., and Adams, J.A. 1996. Biochemistry35, 2022.
Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem.271, 16553.
Adams, J.A., et al. 1995. Biochemistry 34, 2447.
Bosma, M.M., et al. 1993. J. Neurosci.13, 5242.
Brindle, P., et al. 1993. Nature364, 821.
Hajnoczky, G., et al. 1993. Biochem. J.293, 413.
Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA90, 10135.
Wang, L-Y., et al. 1993. Science259, 1173.
Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem.264, 20940.
Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA83, 1300.
Walsh, D.A., et al. 1968. J. Biol. Chem.243, 3763.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable