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407320

γ-Interferon, Mouse, Recombinant, E. coli

Sinónimos:

Interferon γ precursor, IFN-γ, rmIFN-γ

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A ustedes/SKUDisponibilidadPrecio
20 μg
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195,00 €
100 μg
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609,00 €

Acerca de este artículo

NACRES:
NA.77
UNSPSC Code:
12352202
Form:
lyophilized
Assay:
≥95% (RP-HPLC, FPLC, and SDS-PAGE)

195,00 €


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assay

≥95% (RP-HPLC, FPLC, and SDS-PAGE)

Quality Segment

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated

solubility

soluble (Reconstitute with sterile water to >=100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.)

shipped in

wet ice

storage temp.

−20°C

General description

Recombinant, mouse γ-interferon (γ-IFN) expressed in E. coli as a single, non-glycosylated polypeptide containing 134 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.

Biochem/physiol Actions

Reported as IU/mg on a lot-specific basis using a cytopathic effect inhibition assay.

Physical form

Lyophilized from 0.5X PBS, pH 7.2.

Preparation Note

Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for 2-7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
Reconstitute with sterile water to ≥100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.

Other Notes

Alvarado, J., et al. 2005. Immunol. Lett.99, 109.
Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med.43, 499.
Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal.19, 128.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Este artículo
407293407306GF305
assay

≥95% (RP-HPLC, FPLC, and SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

-

assay

>98% (SDS-PAGE and HPLC)

form

lyophilized

form

liquid

form

liquid

form

-

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

storage condition

OK to freeze, desiccated

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

-

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

storage temp.

-10 to -25°C

solubility

soluble (Reconstitute with sterile water to >=100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.)

solubility

-

solubility

-

solubility

-


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Preguntas

  1. How can product 407320-20UG be measured in IU for a dose concentration of 100 IU/mL for an experiment, considering that the label specifies a specific activity of 10 million U/mg P? Is there any interchangeability between these units and the ones I require?

    1 respuesta
    1. The activity is assessed in "IU" using an inhibition assay, with the result corresponding to a specific activity in international units per milligram (IU/mg).

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