382159-M

PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb

liquid, Calbiochem^®

• Código UNSPSC: 12352203

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: liquid
• contiene: ≤0.1% sodium azide as preservative
• reactividad de especies: Xenopus, Tetrahymena sp., human, Drosophila, Caenorhabditis elegans
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• isotipo: IgG
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: phosphorylation (pSer10)
• Información sobre el gen: human ... H3C1(8350)

Descripción

Descripción general

Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser¹⁰.

Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser¹⁰.

This PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb is validated for use in Immunoblotting, ICC, IHC, IP for the detection of Histone H3 (pSer¹⁰) (7-20).

Inmunógeno

Human

a synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH

Aplicación

Immunoblotting (0.5 µg/ml)

Immunocytochemistry (5 µg/ml)

Immunohistochemistry (see comments)

Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)

Advertencia

Toxicity: Standard Handling (A)

Forma física

In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.

Reconstitución

Following initial thaw, aliquot and freeze (-20°C).

Nota de análisis

Positive Control
Acid-extracted proteins from colcemid-arrested HeLa cells

Otras notas

Ajiro, K., et al. 1996. J. Biol. Chem.271, 13197.
Chadee, D.N., et al. 1995. J. Biol. Chem.270, 20098.
Mahadevan, L.C., et al. 1991. Cell65, 775.

For immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable