251515-M

DAF-FM

A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

• Sinónimos: DAF-FM, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein
• Fórmula empírica (notación de Hill): C₂₁H₁₄F₂N₂O₅
• Número de CAS: 254109-20-1
• Peso molecular: 412.34
• Código UNSPSC: 12352200

Propiedades

• Nivel de calidad: 100
• Análisis: ≥98% (HPLC)
• formulario: solution
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; protect from light
• color: pale yellow
• fluorescencia: λ_ex ~500 nm; λ_em ~515 nm
• temp. de almacenamiento: −20°C
• InChI: 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
• Clave InChI: DIJCILWNOLHJCG-UHFFFAOYSA-N

Descripción

Descripción general

A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Reacts with NO in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8.

A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

Acciones bioquímicas o fisiológicas

Primary Target
Nitric oxide (NO) fluorescent indicator

Advertencia

Toxicity: Irritant (B)

Forma física

In DMSO.

Reconstitución

Following initial thaw, aliquot and freeze at -20°C. Use immediately after dilution, discard any unused portion.

Otras notas

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew. Chem. Int. Ed.38, 3209.

Información legal

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): 188.6 °F
• Punto de inflamabilidad (°C): 87 °C