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Magna ChIP® G - Chromatin Immunoprecipitation Kit

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About This Item

Código UNSPSC:
41105331
NACRES:
NA.84

descripción

22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic G beads.

Nivel de calidad

100

envase

kit of 22 assay(s)

técnicas

immunoprecipitation (IP): suitable

Descripción general

Chromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.

Características y beneficios

  • Faster: Magnetic protein G beads allow for the entire ChIP protocol to be done in as little as a day! All reagents to process your samples are included - you don′t have to spend valuable time making them.
  • Easier: Spin columns make DNA purification easier and more reliable - no more messy phenol-chloroform extractions.

Envase

Two boxes containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Componentes

  • Magnetic Protein G Beads
  • ChIP Dilution Buffer
  • Low Salt Wash Buffer
  • High Salt Wash Buffer
  • LiCl Wash Buffer
  • TE Buffer
  • Cell Lysis Buffer
  • Nuclear Lysis Buffer
  • ChIP Elution Buffer (w/o Proteinase K)
  • 10X Glycine
  • 10X PBS
  • Protease Inhibitor Cocktail II
  • Proteinase K
  • Spin Filters
  • Collection Tubes
  • Bind Reagent A
  • Wash Reagent B
  • Elution Reagent C

Almacenamiento y estabilidad

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.

Información legal

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogramas

FlameExclamation mark
GHS02,GHS07

Palabra de señalización

Danger

Frases de peligro

H225,H302,H315,H319,H412

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2

Código de clase de almacenamiento

3 - Flammable liquids

Punto de inflamabilidad (°F)

55.4 °F

Punto de inflamabilidad (°C)

13 °C

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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