14-752-M

GRK7 Protein, active, 10 µg

• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Propiedades

• origen biológico: human
• Nivel de calidad: 100; 300
• mol peso: M_w 66 kDa
• fabricante / nombre comercial: Upstate^®
• técnicas: activity assay: suitable (kinase)
• Nº de acceso NCBI: NM_139209.2
• Nº de acceso UniProt: Q8WTQ7

Descripción

Descripción general

N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.

Recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.

Aplicación

Active, N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells. For use in Kinase Assays.

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease|Metabolic Disorders

Acciones bioquímicas o fisiológicas

• Protein Target: GRK7
• Target Sub-Family:AGC

Calidad

Routinely evaluated by phosphorylation of 2 mg/mL casein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of GRK7.

Definición de unidad

Specific Activity: where one unit of GRK7 activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30°C with a final ATP concentration of 100μM.

Forma física

10μg of recombinant protein in 100 μl of 50 mM Tris-HCl pH 8.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Format: Purified using Ni²⁺/NTA agarose. Purity 99% by SDS-PAGE and Coomassie blue staining.

Almacenamiento y estabilidad

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H317
• Consejos de prudencia: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Clasificaciones de peligro: Skin Sens. 1
• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable