14-744-M

Haspin Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells. For use in Kinase Assays.

• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Propiedades

• origen biológico: human
• Nivel de calidad: 100; 300
• recombinante: expressed in baculovirus infected Sf21 cells
• actividad específica: 2237 U/mg
• mol peso: M_w 41.1 kDa
• fabricante / nombre comercial: Upstate^®
• técnicas: activity assay: suitable (kinase)
• Nº de acceso NCBI: NM_031965.2
• Nº de acceso UniProt: Q8TF76

Descripción

Descripción general

N-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells.

Product Source: Recombinant enzyme expressed in Sf21 cells

Aplicación

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Acciones bioquímicas o fisiológicas

Protein Target: haspin

Target Sub-Family: Misc

Calidad

Routinely evaluated by phosphorylation of 500 μM RARTLSFAEPG.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of Haspin.

Definición de unidad

Specific Activity: where one unit of Haspin activity is defined as 1 nmol phosphate incorporated into 500 μM RARTLSFAEPG per minute at 30°C with a final ATP concentration of 100 μM.

Forma física

10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Purified using Ni²⁺/NTA agarose. Purity 83% by SDS-PAGE and Coomassie blue staining.

Almacenamiento y estabilidad

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H317
• Consejos de prudencia: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Clasificaciones de peligro: Skin Sens. 1
• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable