14-743-M

GCK Protein, active, 10 µg

Active, N-terminal GST-tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells. For use in Kinase Assays.

• Sinónimos: germinal center kinase
• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Propiedades

• origen biológico: human
• Nivel de calidad: 100
• recombinante: expressed in baculovirus infected Sf21 cells
• actividad específica: 2337 U/mg
• mol peso: M_w 80.6 kDa
• fabricante / nombre comercial: Upstate^®
• técnicas: activity assay: suitable (kinase)
• Nº de acceso NCBI: NM_004579.2
• Nº de acceso UniProt: Q12851

Descripción

Descripción general

N-terminal GST- tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells.

Product Source: Recombinant protein expressed in Sf21 cells

Aplicación

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Acciones bioquímicas o fisiológicas

Protein Target: GCK

Target Sub-Family: STE

Calidad

Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active GCK.

Definición de unidad

Specific Activity: where one unit of GCK activity is defined as 1nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.

Forma física

Purified using glutathione-agarose. Purity 52% by SDS-PAGE and Coomassie blue staining.

Almacenamiento y estabilidad

Stable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.