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14-557-M

Sigma-Aldrich

B-Raf (V599E) Protein, active, 10 µg

Active, N-terminal GST-tagged recombinant human B-Raf residues 416-end, containing a V599E mutation, for use in Kinase Assays.

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About This Item

Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.41

origen biológico

human

Nivel de calidad

recombinante

expressed in baculovirus infected Sf21 cells

mol peso

Mw 67.3 kDa

fabricante / nombre comercial

Upstate®

técnicas

activity assay: suitable (kinase)

Nº de acceso NCBI

Nº de acceso UniProt

Descripción general

N-terminal GST-tagged recombinant human B-Raf residues 416-end, containing a V599E mutation (also designated V600E)
Product Source: Expressed by baculovirus in Sf21 insect cells

Aplicación

Research Category
Inflammation & Immunology

Acciones bioquímicas o fisiológicas

Protein Target: B-Raf
Target Sub-Family: TKL

Envase

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-557M when ordering the 250 µg size.

Calidad

routinely evaluated by phosphorylation of MBP substrate in vitro

Forma física

Glutathione agarose affinity chromatography

Almacenamiento y estabilidad

6 months at -70°C

Otras notas

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Melanoma is a form of skin cancer that has a poor prognosis and which is on the rise in Western populations. If detected early, it is easily treated by surgical excision. However, once melanoma metastasises it is notoriously resistant to
Kathryn E Mercer et al.
Biochimica et biophysica acta, 1653(1), 25-40 (2003-06-05)
A recent report has shown that activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations represent a single nucleotide change
Stevan R Hubbard
Cell, 116(6), 764-766 (2004-03-24)
A study by Wan et al. in this issue of Cell demonstrates that the majority of oncogenic mutations in the B-Raf protein kinase result in increased catalytic activity, through disruption of the autoinhibited state of the kinase domain. Surprisingly, several

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