14-484-M

PKCα Protein, active, 10 µg

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

• Sinónimos: PRKCA, Protein kinase C alpha type
• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Propiedades

• origen biológico: human
• Nivel de calidad: 100
• recombinante: expressed in baculovirus infected Sf21 cells
• Línea del producto: Upstate^®
• actividad específica: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• caducidad: 1 yr
• mol peso: M_w 78 kDa
• purificado por: (Ni²⁺/NTA-agarose)
• reactividad de especies: human
• fabricante / nombre comercial: Upstate^®
• técnicas: activity assay: suitable (kinase)
• nº de acceso: (FUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. ); NM_002737.2
• Nº de acceso NCBI: NM_002737.2
• Nº de acceso UniProt: P17252
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... PRKCA(5578)

Descripción

Descripción general

Research area: Apoptosis

Protein Target: PKCα

Target Sub-Family: AGC

Recombinant, full-length human PKCα, containing N-terminal His6 tag

Aplicación

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

Acciones bioquímicas o fisiológicas

Protein kinase C-α (PKCα), an isoform of protein kinase C, exhibits an anti-apoptotic and pro-proliferative function. It is commonly upregulated in tumor cells, and its depletion has been associated with apoptosis induction. The anti-apoptotic effects of PKCα are mediated through mechanisms such as phosphorylation of the anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) and activation of the pro-survival protein kinase AKT. The inhibition of PKCα leads to the activation of a PKCδ-dependent apoptotic pathway in salivary epithelial cells. PKCα regulates cell cycle progression in glioma cells by increasing the expression of p21 cyclin-dependent kinase inhibitor 1 (p21 (Waf1/Cip1)), a protein involved in cell cycle regulation.

Envase

Also available in 10 μg size and in bulk

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-484M when ordering the 250 µg size.

Calidad

routinely evaluated by phosphorylation of Histone H1.

Descripción de destino

PKCα

Forma física

Ni²⁺/NTA-agarose

Almacenamiento y estabilidad

1 year at -70°C

Otras notas

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H317
• Consejos de prudencia: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Clasificaciones de peligro: Skin Sens. 1
• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°C): Not applicable