1.11662

CHAPS

for biochemistry

• Sinónimos: CHAPS, 3-[(3-Cholamidopropyl)dimethylammonio] propane sulfonate), 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
• Fórmula empírica (notación de Hill): C₃₂H₅₈N₂O₇S
• Número de CAS: 75621-03-3
• Peso molecular: 614.88
• Número MDL: MFCD00012116
• Código UNSPSC: 12352107
• NACRES: NA.05

Propiedades

• product name: CHAPS (3-[(3-Cholamidopropyl)-dimethylammonio]-propane- sulfonate), for biochemistry
• Nivel de calidad: 100
• formulario: solid
• mol peso: micellar avg mol wt 6150
• número de agregación: 10
• pH: 6 (100 g/L in H₂O)
• CMC: 6 mM (20-25°C)
• mp: 157 °C
• temperatura de transición: cloud point >100 °C
• temp. de almacenamiento: 2-8°C
• cadena SMILES: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• Clave InChI: UMCMPZBLKLEWAF-RFCNGIAKSA-N

Descripción

Aplicación

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

Nota de análisis

Assay (ex N): ≥ 99 %
Identity (IR-spectrum): passes test
Absorbance A (280 nm; 1 %; 1 cm; water): ≤ 0.1
Absorbance A (260 nm; 1 %; 1 cm; water): ≤ 0.1

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H302
• Consejos de prudencia: P264 - P270 - P301 + P312 - P501
• Clasificaciones de peligro: Acute Tox. 4 Oral
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable