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08-110

Sigma-Aldrich

ECL Cell Attachment Matrix

Sinónimos:

ECL Matrix

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About This Item

Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.75

origen biológico

mouse

Nivel de calidad

100

formulario

liquid

fabricante / nombre comercial

Upstate®

técnicas

cell culture | mammalian: suitable

entrada

sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

ECL Cell Attachment Matrix from Engelbreth-Holm-Swarm (EHS) mouse tumor.

Aplicación

Cell culture attachment factor

Calidad

Routinely evaluated in a Neurite Outgrowth Assay using PC12 rat pheochromocytoma cells.

Forma física

5 mg in 5 ml of 0.05 M Tris-HCl, pH 7.4, 0.15 M NaCl. Protein determined by the Bradford dye binding assay using gamma globulin as the standard. Frozen Solution.

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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F B Gertler et al.
Cell, 87(2), 227-239 (1996-10-18)
Drosophila Enabled is required for proper formation of axonal structures and is genetically implicated in signaling pathways mediated by Drosophila AbI. We have identified two murine proteins, Mena and Evl, that are highly related to Enabled as well as VASP
B B Friday et al.
The Journal of cell biology, 149(3), 657-666 (2000-05-03)
Differentiation of skeletal muscle myoblasts follows an ordered sequence of events: commitment, cell cycle withdrawal, phenotypic differentiation, and finally cell fusion to form multinucleated myotubes. The molecular signaling pathways that regulate the progression are not well understood. Here we investigate
Basement membrane complexes with biological activity.
Kleinman, H K, et al.
Biochemistry, 25, 312-318 (1986)
Alteration of Ca2+ dependence of neurotransmitter release by disruption of Ca2+ channel/syntaxin interaction
Rettig, J., et al
The Journal of Neuroscience, 17, 6647-6656 (1997)
Extracellular matrix regulates Sertoli cell differentiation, testicular cord formation, and germ cell development in vitro.
Hadley, M A, et al.
The Journal of cell biology, 101, 1511-1522 (1985)
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Protocolos

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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