07-2101-M

Anti-phospho PIX Antibody (Ser340)

from rabbit, purified by affinity chromatography

• Sinónimos: Rho guanine nucleotide exchange factor (GEF) 7, PAK-interacting exchange factor beta, guanine nucleotide exchange factor 7, rho guanine nucleotide exchange factor 7, SH3 domain-containing proline-rich protein, p85, PAK3, Beta-Pix, P85COOL1, COOL-1, P85,
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: mouse
• reactividad de especies (predicha por homología): rat (based on 100% sequence homology), human (based on 100% sequence homology)
• técnicas: western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_001106989
• Nº de acceso UniProt: Q9ES28
• modificación del objetivo postraduccional: phosphorylation (pSer340)
• Información sobre el gen: human ... PAK3(5063)

Descripción

Descripción general

p-21-activated kinase (PAK) interacting exchange factor (PIX) is a guanine nucleotide exchange factor. PIX is a necessary component for the recruitment, regulation and activation of Rac1, as it is able to directly bind Rac1 via its SH3 domain. Studies indicate that PIX may also have an active role as a positive regulator of apoptosis, an inducer of membrane ruffling and in cell migration. Along with Rac1, PIX has been shown to regulate and interact with GIT1 and NOD2. Alternative splicing and initiation give rise to 8 known isoforms. Expression of PIX has been localized to the central nervous system. Isoforms B, C and E have highest expression in the testis and brain.

Especificidad

This antibody recognizes PIX phosphorylated at Ser340.

Inmunógeno

Epitope: Phosphorylated Ser340

KLH-conjugated linear peptide corresponding to mouse PIX phosphorylated at Ser340.

Aplicación

This phospho PIX antibody is validated for use in WB for the detection of the phospho PIX protein.

Calidad

Evaluated by Western Blot in PIX transfected cell lysates.

Western Blot Analysis: A 1:1000 dilution of this antibody detected PIX on 10 µg of Pix transfected cell lysates.

Descripción de destino

~ 75 kDa

Forma física

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide, 1 mg/mL BSA and 30% glycerol.

Nota de análisis

Control
PIX transfected cell lysates

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1