05-904
Anti-HA Tag Antibody
Upstate®, from mouse
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
monoclonal
reactividad de especies
vertebrates (common species)
fabricante / nombre comercial
Upstate®
técnicas
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG3
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Descripción general
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Especificidad
Reactivity with other species has not been confirmed.
Recognizes HA tagged proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.
Inmunógeno
KLH-conjugated, synthetic peptide corresponding to amino acids 98-106 of influenza hemaglutinin.
Aplicación
Detect HA Tag using this Anti-HA Tag Antibody validated for use in IC, IP & WB.
Immunoprecipitation:
5 μg/mL of his antibody has been reported by an independent laboratory to immunoprecipitate recombinant HA-tagged fusion protein.
Immunocytochemistry:
5 μg/mL of this antibody has been reported by an independent laboratory to immunostain HA-tagged fusion protein.
5 μg/mL of his antibody has been reported by an independent laboratory to immunoprecipitate recombinant HA-tagged fusion protein.
Immunocytochemistry:
5 μg/mL of this antibody has been reported by an independent laboratory to immunostain HA-tagged fusion protein.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Calidad
Routinely evaluated by western blot of recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected a recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected a recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.
Descripción de destino
Various
Ligadura / enlace
Replaces: 07-221
Forma física
Format: Purified
Protein A purified
Purified mouse monoclonal IgG3 in buffer containing PBS, pH 7.4 containing 0.05% sodium azide.
Almacenamiento y estabilidad
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Nota de análisis
Control
RIPA lysates from HEK293 cells.
RIPA lysates from HEK293 cells.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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