05-440
Anti-Cbl Antibody, clone 7G10
clone 7G10, Upstate®, from mouse
Sinónimos:
Cas-Br-M (murine) ecotropic retroviral transforming sequence, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-CBL, RING finger protein 55, Signal transduction protein CBL, oncogene CBL2
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
7G10, monoclonal
reactividad de especies
mouse, rat, human
fabricante / nombre comercial
Upstate®
técnicas
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG1κ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... CBL(867)
Descripción general
Casitas B-lineage lymphoma (Cbl), is an oncogene which causes pre B-cell lymphomas. It participates in the regulation of cell signaling and has the ability to interact with many proteins such as receptor/non-receptor PTK′s, structural proteins, and novel proteins that are not well understood. Diseases associated with Cbl are autoimmune diseases, diabetes, and leukemia. By modifying Cbl′s ability to bind to oncogenic proteins, Cbl may be developed into an anti-cancer tool and in the near future used in therapeutic intervention.
Especificidad
Antibody recognizes amino acids 878-906 on human Cbl
Inmunógeno
Epitope: a.a. 878-906
GST fusion protein corresponding to residues 878-906 of human Cbl
Aplicación
Immunocytochemistry:
5-10 μg/mL of a previous lot showed positive immunostaining for Cbl in 3T3/A31 cells fixed with ice cold Methanol/Ethanol [1:1] for 1 minute.
Immunoprecipitation:
2 μg of a previous lot immunoprecipitated Cbl from 1 mg of a mouse 3T3/A31 cell RIPA lysate, as reported by an independent laboratory.
5-10 μg/mL of a previous lot showed positive immunostaining for Cbl in 3T3/A31 cells fixed with ice cold Methanol/Ethanol [1:1] for 1 minute.
Immunoprecipitation:
2 μg of a previous lot immunoprecipitated Cbl from 1 mg of a mouse 3T3/A31 cell RIPA lysate, as reported by an independent laboratory.
Research Category
Protein Trafficking
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Ubiquitin & Ubiquitin Metabolism
This Anti-Cbl Antibody, clone 7G10 is validated for use in IP, WB, IC for the detection of Cbl.
Calidad
routinely evaluated by immunoblot on RIPA lysates from human Jurkat, mouse 3T3/A31, TF-1, or FDC-P1/Mac11
Descripción de destino
120kDa
Forma física
Format: Purified
Protein G Chromatography
Purified IgG1k in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4
Almacenamiento y estabilidad
Stable for 1 year at 2-8ºC from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Nota de análisis
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Sabine A Bisson et al.
Oncogene, 21(23), 3677-3687 (2002-05-29)
The c-Cbl proto-oncogene acts as an E3 ubiquitin ligase via its RING finger domain to negatively regulate activated cellular signal transduction pathways. We have identified an aberrant Cbl-protein of approximately 95 kDa, which we have called p95Cbl, from the murine
Karisa C Schreck et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 27(22), 6197-6208 (2021-08-27)
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Hua-Bei Guo et al.
Glycobiology, 19(5), 547-559 (2009-02-20)
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Shanshan Yin et al.
The Journal of biological chemistry, 288(37), 26448-26462 (2013-07-31)
T cell antigen receptor γδ (TCRγδ) and natural killer group 2, member D (NKG2D) are two crucial receptors for γδT cell cytotoxicity. Compelling evidences suggest that γδT cell cytotoxicity is TCRγδ-dependent and can be co-stimulated by NKG2D. However, the molecular
T J Blake et al.
Oncogene, 7(4), 757-762 (1992-04-01)
The murine Cas NS-1 retrovirus carries the v-cbl oncogene and induces pro-B, pre-B and myeloid tumors in mice inoculated at birth. The human homolog of v-cbl is located on chromosome 11q23.3, which is in the region of translocation breakpoints in
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