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05-382

Sigma-Aldrich

Anti-Na+/K+ ATPase β-1 Antibody, clone C464.8

clone C464.8, Upstate®, from mouse

Sinónimos:

Anti-AHC2, Anti-ATP1A1, Anti-CAPOS, Anti-DEE99, Anti-DYT12, Anti-RDP

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

C464.8, monoclonal

reactividad de especies

rabbit, bovine, canine, human, rat

fabricante / nombre comercial

Upstate®

técnicas

immunohistochemistry: suitable
western blot: suitable

isotipo

IgG2aκ

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... ATP1A3(478)

Descripción general

Clone C464.8 is also referred to as clone 8A

Especificidad

Recognizes the β-1 subunit isoform of Na+/K+.

Inmunógeno

Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.

Aplicación

Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
This Anti-Na+/K+ ATPase β-1 Antibody, clone C464.8 is validated for use in IH, WB for the detection of Na+/K+ ATPase β-1.

Calidad

routinely evaluated in immunoblot on rat brain, rat heart or rat kidney microsomal preparations

Descripción de destino

broad band at an apparent molecular weight of 48-55 kDa due to glycosylation

Forma física

Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in PBS with 0.05% sodium azide before the addition of glycerol to 30%

Almacenamiento y estabilidad

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análisis

Control
Expressed in most tissue, including brain, heart and kidney

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Aude Belliard et al.
Physiological reports, 4(19) (2016-10-06)
Cardiac glycosides (CG) are traditionally known as positive cardiac inotropes that inhibit Na+/K+-ATPase-dependent ion transport. CG also trigger-specific signaling pathways through the cardiac Na+/K+-ATPase, with beneficial effects in ischemia/reperfusion (I/R) injury (e.g., ouabain preconditioning, known as OPC) and hypertrophy. Our
Analysis of subunit assembly of the Na-K-ATPase.
Fambrough, D M, et al.
The American Journal of Physiology, 266, C579-C589 (1994)
Motor neuron degeneration in amyotrophic lateral sclerosis mutant superoxide dismutase-1 transgenic mice: mechanisms of mitochondriopathy and cell death.
Lee J Martin,Zhiping Liu,Kevin Chen,Ann C Price,Yan Pan,Jason A Swaby,W Christopher Golden
The Journal of Comparative Neurology null
Regional distribution of Na,K-ATPase activity in porcine lens epithelium
Tamiya, S., et al
Investigative Ophthalmology & Visual Science, 44, 4395-4399 (2003)
J Codina et al.
The Journal of biological chemistry, 273(14), 7894-7899 (1998-05-09)
Previous experiments from our laboratory (Codina, J., Kone, B. C., Delmas-Mata, J. T., and DuBose, T. D., Jr. (1996) J. Biol. Chem. 271, 29759-29763) demonstrated that the alpha-subunit of the colonic H+, K+-ATPase (HKalpha2) requires coexpression with a beta-subunit to

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