05-212
Anti-PI3 Kinase Antibody, p85, N-SH3, clone AB6
clone AB6, Upstate®, from mouse
Sinónimos:
PI3-kinase p85 subunit alpha, PtdIns-3-kinase p85-alpha, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85
alpha), phosphatidylinositol 3-kinase, regulatory, 1, phosphatidylinositol 3-kinase-associated p-85 alpha, phosphoinos
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
AB6, monoclonal
reactividad de especies
human, rat, mouse
fabricante / nombre comercial
Upstate®
técnicas
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG1κ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... PIK3R1(5295)
Descripción general
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that is thought to phosphorylate second messengers in growth signaling pathways. PI3K exists as a heterodimer of catalytic 110 kDa (p110) and regulatory 85 kDa (p85, p50 or p55) subunits. The p85 unit consists of two closely related proteins: p85 alpha, which binds tightly to the catalytic subunit and p85 beta, a protein of unknown function. They share 62% amino acid sequence identity. p85 alpha lacks PI3 kinase activity, but may serve as regulator of the catalytic subunit, p110, by acting as the link between PI3K and the ligand activated receptor. Both isoforms bind to activated receptors and serve as tyrosine kinase substrates.
Especificidad
This antibody recognizes p85 alpha subunit of PI3 Kinase; does not recognize p85 beta subunit.
Inmunógeno
Recombinant human p85 alpha PI3 Kinase expressed in E. Coli. Clone AB6.
Aplicación
Immunocytochemistry:
10 µg/mL of a previous lot of this antibody was used in immunocytochemistry.
Immunoprecipitation:
4 µg of a previous lot immunoprecipitated PI3 Kinase from 500 µg of an A431 RIPA lysate, as confirmed by immunoblot using a polyclonal PI3 Kinase antibody (Catalog # 06-195).
10 µg/mL of a previous lot of this antibody was used in immunocytochemistry.
Immunoprecipitation:
4 µg of a previous lot immunoprecipitated PI3 Kinase from 500 µg of an A431 RIPA lysate, as confirmed by immunoblot using a polyclonal PI3 Kinase antibody (Catalog # 06-195).
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
Kinases & Phosphatases
PI3K, Akt, & mTOR Signaling
Kinases & Phosphatases
This Anti-PI3 Kinase Antibody, p85, N-SH3, clone AB6 is validated for use in IC, IP, WB for the detection of PI3 Kinase.
Calidad
Routinely evaluated by Western Blot on RIPA lysates from human A431 cells, or RIPA lysates from rat L6 myoblasts.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected PI3 Kinase in RIPA lysates from human A431 cells; previous lots detected PI3 Kinase in RIPA lysates from rat L6 myoblasts.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected PI3 Kinase in RIPA lysates from human A431 cells; previous lots detected PI3 Kinase in RIPA lysates from rat L6 myoblasts.
Descripción de destino
85 kDa
Ligadura / enlace
Replaces: 04-403
Forma física
Format: Purified
Protein A chromatography
Purified mouse monoclonal IgG1κ in buffer containing PBS, pH 7.2 with 50% glycerol.
Almacenamiento y estabilidad
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.
Nota de análisis
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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