04-1475-I

Anti-CD27 Antibody, clone RM27-3E5

clone RM27-3E5, from rat

• Sinónimos: CD27 antigen, CD27, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: RM27-3E5, monoclonal
• reactividad de especies: mouse
• técnicas: flow cytometry: suitable; neutralization: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_001028298
• Nº de acceso UniProt: P41272
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Cd27(21940)

Descripción

Descripción general

CD27 antigen (UniProt P41272; also known as CD27, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7) is encoded by the Cd27 (also known as Tnfrsf7) gene (Gene ID 21940) in murine species. CD27 is a TNF receptor superfamily member that binds to CD70 and mediates the survival, function, and differentiation of T-, B-, natural killer (NK), and plasma cells. Interaction with CD70 provides costimulation in T cells induces signaling events essential for cell proliferation, long-term maintenance of antigen-specific T cells, antiviral responses, antitumor immunity, and alloreactivity. CD27 ligation on B-cells by CD70 causes enhanced plasma cell formation and increased IgG production. CD27 is also used as a marker for memory B cells in the classification of B-cell deficiencies, including common variable immunodeficiency (CVID). CD27 is synthesized with a signal peptide (a.a. 1-23), which was removed posttranslationally to yield the mature type I transmembrane protein with an extracellular (a.a. 24-182) composed of three TNFR-Cys repeats (a.a. 26-63, 64-104, 105-141), followed by a transmembrane region (a.a. 183-203), and a cytoplasmic tail (a.a. 204-250).

Especificidad

Clone RM27-3E5 enhanced anti-CD3-stimulated T-cell proliferation when immobilized in vitro and augmented OVA-specific CTL induction when administrated in vivo (Roberts, D.J., et al. (2010). J. Immunother. 33(8): 769 779; Sumi, T., et al. (2008). Immunol. Lett. 119(1-2):91-96).

Inmunógeno

Epitope: extracellular domain

Human IgG1 Fc-tagged recombinant mouse CD27 extracellular domain (Sumi, T., et al. (2008). Immunol. Lett. 119(1-2):91-96).

Aplicación

Neurtralizing Analysis: A representative lot, when injected intraperitoneally following OVA-expressing B16 melanoma cells (B16-cOVA) implant, suppressed B16-cOVA lung metastasis and tumor growth among wild-type, but not Cd27-knockout, mice (Roberts, D.J., et al. (2010). J. Immunother. 33(8): 769 779).

Neurtralizing Analysis: A representative lot, when injected intraperitoneally during the induction phase of ragweed-induced experimental allergic conjunctivitis (EC), significantly affected the systemic immune responses, but not the severity of EC in mice (Sumi, T., et al. (2008). Immunol. Lett. 119(1-2):91-96).

Research Category
Inflammation & Immunology

This rat monoclonal Anti-CD27 Antibody, clone RM27-3E5, Cat. No. 04-1475-I is validated for use in Flow Cytometry and Neutralization applications for the analysis of CD27.

Calidad

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 2 µL of this antibody detected CD27-positive mouse splenocytes.

Descripción de destino

25.69/28.16 kDa (mature/pro-form) calculated.

Ligadura / enlace

Replaces: 04-1475

Forma física

Format: Purified

Protein G purified.

Purified rat IgG2a in PBS without preservatives.

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable