04-037
Anti-RalB Antibody, clone 25
clone 25, Upstate®, from mouse
Sinónimos:
RAS-like protein B, v-ral simian leukemia viral oncogene homolog B, GTP binding protein, v-ral simian leukemia viral oncogene homolog B
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
25, monoclonal
reactividad de especies
mouse, rat, human
fabricante / nombre comercial
Upstate®
técnicas
western blot: suitable
isotipo
IgG1κ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... RALB(5899)
Descripción general
RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarizatied exocystossis in epithelaial cells (Cascone, 2008). RalB is required for metastatic formation and cell survival by driving exocyst assembly during polarized cell migration (Cascone, 2008). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes
Especificidad
Other species have not been tested.
This antibody is specific for RalB and does not detect RalA.
Inmunógeno
Epitope: C-terminus
Synthetic peptide of the C-terminus of RalB
Aplicación
Detect RalB using this Anti-RalB Antibody, clone 25 validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
G-proteins
Cytoskeletal Signaling
G-proteins
Calidad
Evaluated by western blot on HEK293 or NIH/3T3 cell lysates.
Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect RalB in HEK293, NIH/3T3, and rat liver cell lysate.
Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect RalB in HEK293, NIH/3T3, and rat liver cell lysate.
Descripción de destino
24 kDa
Forma física
Format: Purified
Protein G Purified
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Almacenamiento y estabilidad
Stable for 1 year at 2-8ºC from date of receipt.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Genetic Deletion of RALA and RALB Small GTPases Reveals Redundant Functions in Development and Tumorigenesis.
Pascal Peschard,Afshan McCarthy,Val Leblanc-Dominguez,Maggie Yeo,Sabrina Guichard et al.
Current Biology null
Masayuki Uegaki et al.
Carcinogenesis, 40(12), 1535-1544 (2019-05-07)
RalGTPase-activating protein (RalGAP) is an important negative regulator of small GTPases RalA/B that mediates various oncogenic signaling pathways in various cancers. Although the Ral pathway has been implicated in prostate cancer (PCa) development and progression, the significance of RalGAP in
Carine Rossé et al.
Molecular and cellular biology, 26(2), 727-734 (2005-12-31)
The Ras family GTPases RalA and RalB have been defined as central components of the regulatory machinery supporting tumor initiation and progression. Although it is known that Ral proteins mediate oncogenic Ras signaling and physically and functionally interact with vesicle
Richard R Clough et al.
The Journal of biological chemistry, 277(32), 28972-28980 (2002-05-30)
Ral GTPases may be involved in calcium/calmodulin-mediated intracellular signaling pathways. RalA and RalB are activated by calcium, and RalA binds calmodulin in vitro. It was examined whether RalA can bind calmodulin in vivo, whether RalB can bind calmodulin, and whether
Hua Yang et al.
The Journal of biological chemistry, 295(10), 3055-3063 (2020-02-01)
In human cancer cells that harbor mutant KRAS and WT p53 (p53), KRAS contributes to the maintenance of low p53 levels. Moreover, KRAS depletion stabilizes and reactivates p53 and thereby inhibits malignant transformation. However, the mechanism by which KRAS regulates
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico