03-0206-00

SMC^® Human SNAP-25 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human SNAP-25 in human serum, plasma, and cerebrospinal fluid (CSF)

• Sinónimos: SNAP-25 Single Molecule Counting kit, SNAP-25 immunoassay kit, SNAP-25 ultra-sensitive immunoassay, Synaptosomal-associated protein 25 immunoassay kit
• NACRES: NA.77
• UNSPSC Code: 12161503
• Species reactivity: human
• Technique(s): single molecule counting: suitable
• Usage: kit sufficient for 96 wells
• Compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments

Propiedades

• Nombre del producto: SMC^® Human SNAP-25 High Sensitivity Immunoassay Kit,
• Quality Segment: 200
• description: Used for ultrasensitive quantification of Human SNAP-25 in human serum, plasma, and cerebrospinal fluid (CSF)
• product line: SMC^®
• usage: kit sufficient for 96 wells
• species reactivity: human
• technique(s): single molecule counting: suitable
• input: serum; plasma (K2 EDTA); cerebrospinal fluid (CSF)
• UniProt accession no.: P60880
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments
• shipped in: wet ice
• storage temp.: 2-8°C

Descripción

General description

Synaptosomal-associated protein 25 (SNAP-25) plays a crucial role in synaptic transmission in the nervous system. As a component of the SNARE complex, it facilitates the fusion of synaptic vesicles with the presynaptic membrane, enabling the release of neurotransmitters into the synaptic cleft. By promoting efficient neurotransmitter release, SNAP-25 is essential for proper neuronal communication and signaling.

SNAP-25 has been implicated in various conditions, including Alzheimer′s disease, schizophrenia, and multiple sclerosis. Abnormalities in SNAP-25 expression or function can disrupt synaptic transmission, leading to impaired neuronal communication and contributing to cognitive decline and other symptoms associated with these disorders. Alterations in SNAP-25 levels often correlate with disease severity, highlighting its importance in neuroinflammation and synaptic dysfunction. Understanding the role of SNAP-25 in these processes can help identify potential therapeutic targets and inform strategies aimed at restoring synaptic function and improving outcomes for neurological disorders.

Application

Alzheimer′s Disease / Aging / Nervous System / Neurodegenerative Diseases / Neurofilament & Neuron Metabolism / Neuronal & Glial Markers / Neurodegenerative Medicine / Neuroscience

Features and Benefits

• Ultra-Sensitive Measurement: Accurately detects SNAP-25 at femtogram per milliliter levels in serum, plasma and CSF.
• Reliable Data: An improved signal-to-noise ratio extends the dynamic range to guarantee precise and trustworthy assay results.
• Easy to Adopt: Follow a well-known sandwich immunoassay protocol to seamlessly incorporate SMC^®technology into research processes.
• Automated Workflow: Designed for compatibility with automated liquid handling systems, this assay minimizes manual tasks and enhances laboratory efficiency.
• Expert Support: Get technical assistance from specialized field scientists and technical teams.

Packaging

96 well assay

Analysis Note

• Sensitivity: LLOQ 260 fg/mL* (SMCxPRO^® Instrument)
• Standard Curve Range: 130-300,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Serum: 112% Plasma: 110% CSF: 97%
• Recovery: Serm: 82% Plasma: 91% CSF: 89%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although SNAP-25 was detectable in non-human primate (rhesus macaque and cynomolgus monkey), feline, rat, mouse, canine samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302 + H332,H311,H319,H360FD,H412,EUH430
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• pcodes: P201 - P202 - P273 - P280 - P308 + P313 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B
• Clase de almacenamiento: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects