WHA10402578
Whatman® Protran® nitrocellulose membranes
Circles - BA85, pore size 0.45 μm, diam. 25 mm, pack of 1000 ea
Sinónimos:
Z670642, blotting membranes, nitrocellulose membrane, transfer membranes
About This Item
Productos recomendados
envase
pack of 1000 ea
fabricante / nombre comercial
Whatman 10402578
Whatman Article No. 28415104 (US reference)
Diámetro
25 mm
tamaño de poro
0.45 μm pore size
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Descripción general
- Binding capacity, 75 - 110 μg/cm2
- High binding, low background
- Triton free matrix
- Universal membrane for blotting - isotopic, chemluminescent, colorimetric and fluorescent detection methods
Protran nitrocellulose (NC) membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as ‘nitrocellulose′ may actually contain large amounts of cellulose acetate which will lower the protein binding capacity. Protran membranes have the best handling strength of all pure nitrocellulose membranes.
They are compatible with a variety of detection methods, including isotopic, chemiluminescent (luminol-based), colorimetric and fluorescent. Unlike PVDF membranes, Protran nitrocellulose does not require a methanol pre-wetting step. This makes Protran the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wetted in water, and then placed in the transfer buffer. No other pre-treatment steps are necessary.
High Binding, Low Background:
In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions.
High Retention of Small Proteins:
The 0.2 μm pore size of the Protran (BA83) nitrocellulose membrane has high surface area, ensuring binding of small proteins below 20 kD by reducing ‘blow-through′. The 0.45 μm pore size membrane (BA85) is ideal for larger molecular weight samples. BA79, with 0.1 μm pore size, is the membrane of choice for smaller proteins below 7 kD. A unique benefit of the proprietary Protran nitrocellulose formula is the proven shelf life of bound proteins. Empirical evidence shows that proteins maintain molecular recognition activity for five years on Protran.
Protran Blotting Sandwiches:
A pre-cut nitrocellulose membrane and 2 sheets of 3MM Chr blotting paper are prepackaged into a sandwich to help save time. This is made with our Protran BA83 or BA85, the highest quality NC membranes available for blotting.
Otras notas
Información legal
Sold under license to DE10102744 and foreign equivalents thereof
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Flam. Sol. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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