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G005684

HPLC Analysis of Caffeine Metabolites and Analogs on Discovery® C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Discovery C18, 15 cm x 4.6 mm I.D., 5 μm particles (504955)
mobile phase: [A] 1% acetic acid in water; [B] methanol; (92:8, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 254 nm

suitability

application for HPLC

application(s)

clinical
vitamins, nutraceuticals, and natural products

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    7-Methylxanthine
    1-Methyluric acid
    1-Methylxanthine
    3-Methylxanthine
    1,3-Dimethyluric acid
    Theophylline
    1,7-Dimethylxanthine
    Theobromine
    See All (8)

analytical column

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