TSKgel® Amide-80 HPLC Column, phase carbamoyl, L × I.D. 25 cm × 4.6 mm, 5 μm particle size
material
stainless steel
Agency
suitable for USP L68
product line
TSKgel®
manufacturer/tradename
Tosoh 13071
parameter
0-100% organic solvent 10-80 °C temperature 150 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
450 m2/g
matrix
silica particle platform
matrix active group
carbamoyl phase
particle size
5 μm
pore size
100 Å
operating pH
2.0-7.5
separation technique
hydrophilic interaction (HILIC)
General description
TSKgel Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications.
Physical form
Shipped in 85% acetonitrile - 15% water.
Recommended products
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
