Nothing special is required to use Ascentis® Express F5 HPLC Columns. To obtain the full benefits of Ascentis® Express Columns, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
53567-U
Ascentis® Express 90 Å F5 (2.7 μm) HPLC Columns
L × I.D. 5 cm × 2.1 mm, HPLC Column
Synonym(s):
Core-shell (SPP) Fused Core PFP HPLC column
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€579.00
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1 EA
€579.00
About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
€579.00
Please contact Customer Service for Availability
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Product Name
Ascentis® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 5 cm × 2.1 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L43
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
60 °C temp. range
600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
5 cm × 2.1 mm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform
superficially porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
2.7 μm
pore size
90 Å pore size
operating pH
2-8
application(s)
food and beverages
separation technique
reversed phase
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General description
The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Application
guard cartridge
Product No.
Description
Pricing
related product
required but not provided
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore
Articles
This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.
This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.
Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds
Protocols
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®
Chromatograms
application for HPLC-
Is there anything special I need to do to my HPLC system to use Ascentis® Express F5 HPLC Columns?
1 answer-
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How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
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The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can I use Ascentis® Express F5 HPLC Columns on any type of HPLC system?
1 answer-
Ascentis® Express F5 HPLC Columns HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. These columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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What is the pressure rating for Ascentis® Express F5 HPLC Columns?
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Ascentis® Express F5 HPLC Columns are stable to operating pressures up to 600 bar (9000 psi).
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Do I need special fittings and tubing to connect Ascentis® Express F5 HPLC Columns?
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While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
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Can I use Ascentis® Express F5 HPLC Columns on a UHPLC system?
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Ascentis® Express F5 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis® Express F5 HPLC Columns outperform sub-2 μm micron columns on many applications since Ascentis® Express F5 HPLC Columns provide the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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What flow rate should I use with Ascentis® Express F5 HPLC columns?
1 answer-
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis® Express F5 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
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What is the carbon load for Ascentis® Express F5 HPLC Columns?
1 answer-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express F5 HPLC columns is 3.5 micromoles/meter-squared.
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Are guard columns available for Ascentis® Express F5 2.1 mm ID HPLC Columns?
1 answer-
Yes, we have guard columns available for Ascentis® Express F5 HPLC Column . Product 53500-U is the Ascentis® Express Guard Holder and Product 53594-U Ascentis® Express F5 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 2.1 mm .
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