ZRB2276

Anti-CUX1 Antibody, clone 1J18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): CCAAT displacement protein, CDP, Homeobox protein Cux-1, Homeobox protein cut-like 1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1J18, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 164.19 kDa; observed mol wt ~230 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_853530
• UniProt accession no.: P39880
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... CUX1(1523)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1J18 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Homeobox protein cut-like 1 (CUX1). It targets an epitope within 21 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal half of human Homeobox protein cut-like 1 (CUX1).

Application

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell nuclear extract.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CUX1 in HEK293 cell nuclear extract.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CUX1 in Human cerebellum tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound CUX1 peptide with a KD of 6.7 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CUX1 in K562 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Homeobox protein cut-like 1 (UniProt: P39880; also known as CCAAT displacement protein, CDP, Homeobox protein cux-1, CCAAT displacement protein, CDP, Homeobox protein cux-1) is encoded by the CUX1 (also known as CUTL1) gene (Gene ID: 1523) in human. Homebox protein CUX1 is member of the CUT Homebox family that has a broad role in mammalian development. It is a transcription factor that is involved in the control of neuronal differentiation in the brain. It regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III. It has a conserved coiled-coil region at the N terminus (aa 56-407), three internal 60-amino-acid repeats (Cut repeats; 542-629; 934-1021; and 1117-1204), and a divergent homeodomain located near the C terminus (aa 1244-1303). CUX1 serves as a repressor of developmentally regulated gene expression and acts by preventing binding of positively-acting CCAAT factors to promoters. It is also a component of NF-muNR repressor, which binds to the matrix attachment regions (MARs) (5′ and 3′) of the immunoglobulin heavy chain enhancer. CUX1 is shown to repress T-cell receptor β enhancer function by binding to the matrix attachment region beta (MARβ), which is an ATC-rich DNA sequence located upstream of the TCR β enhancer. Its cleaved product, CDP/Cux p110, is reported to play a role in cell cycle progression, in particular at the G1/S transition. While CUX1 only transiently binds to DNA and carries the CCAAT-displacement activity, CDP/Cux p110 makes a stable interaction with DNA and stimulates expression of genes such as POLA1. Eleven different isoforms of CUX1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Goulet, B., et al. (2004). Mol. Cell. 14(2); 207-219; Ellis, T., et al. (2001). Genes Dev. 15 (17); 2307-2319).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable