V5765
VEGF165
recombinant, expressed in HEK 293 cells, suitable for cell culture
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
Recommended Products
biological source
human
Quality Level
recombinant
expressed in HEK 293 cells
description
Vascular Endothelial Growth Factor 165 human
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
<10 ng/mL ED50
quality
endotoxin tested
mol wt
dimer 42 kDa (glycosylated)
packaging
pkg of 10 μg
pkg of 50 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.075 EU/μg
color
white
solubility
water: soluble
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... VEGFA(7422)
General description
VEGF165 is expressed in human HEK 293 cells as a glycosylated homodimer with an apparent molecular mass of 45 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Biochem/physiol Actions
VEGF165 belongs to the PDGF/VEGF growth factor family characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF is secreted by the majority of tumor cells and initiates angiogenesis by activating endothelial cells of existing blood vessels and promoting their migration.
VEGF has also been implicated in correlation with poor prognosis in breast cancer.
In addition, VEGF is released in rheumatoid arthritis in response to TNF-α, increasing endothelial permeability and stimulating angiogenesis (formation of capillaries).
VEGF has also been implicated in correlation with poor prognosis in breast cancer.
In addition, VEGF is released in rheumatoid arthritis in response to TNF-α, increasing endothelial permeability and stimulating angiogenesis (formation of capillaries).
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (human umbilical vein endothelial cells).
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Lee M Ellis
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C J Robinson et al.
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A O Afuwape et al.
Histology and histopathology, 17(3), 961-972 (2002-08-10)
The expansion of the synovial lining of joints in rheumatoid arthritis (RA), and the subsequent invasion by the pannus of underlying cartilage and bone, necessitates an increase in the vascular supply to the synovium, to cope with the increased requirement
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