western blot: 1:1,000-1:2,000 using cytosolic fraction (S1) of mouse brain or a whole cell extract of human lung carcinoma A549 cell line western blot: 1:5,000-1:10,000 using cytosolic fraction (S1) of rat brain
synthetic peptide corresponding to amino acids 56-76 located near the N-terminus of rat UCH-L1, conjugated to KLH. This sequence is identical in human, mouse, bovine, porcine, and guinea pig UCH-L1. No homology is found with other known UCH-L isoforms.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Membrane proteins are crucial to heart function and development. Here we combine cationic silica-bead coating with shotgun proteomics to enrich for and identify plasma membrane-associated proteins from primary mouse neonatal and human fetal ventricular cardiomyocytes. We identify Tmem65 as a
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