As provided, this product contains 100 units per vial. The mass weight per vial is not determined.
U3383
Urease Buffer Reagent
Synonym(s):
Urea colorimetric determination assay reagent
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1 VIAL
€104.00
About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
€104.00
Please contact Customer Service for Availability
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description
When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.
Quality Level
form
powder
mol wt
545 kDa
storage temp.
2-8°C
General description
Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia. [1]
Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide.[2][3] It is secreted by several bacterial species, invertebrates, fungi, and plants.[3] Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections.[2] two Ni2+ ions bridged by a hydroxyl group and a carbamylated lysine.[3]
Application
Urease Buffer Reagent has been used:
- to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method[4]
- in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells[5]
- to study the metabolic capacity of rat hepatocytes[6]
Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Kang Cheng et al.
Hepatology (Baltimore, Md.), 50(4), 1194-1203 (2009-07-29)
Tracking stem/progenitor cells through noninvasive imaging is a helpful means of assessing the targeting of transplanted cells to specific organs. We performed in vitro and in vivo studies wherein adult human hepatocytes and human fetal liver stem/progenitor cells were labeled
Yuta Enami et al.
Hepatology (Baltimore, Md.), 55(4), 1182-1192 (2011-10-14)
Organs from non-heart-beating donors are attractive for use in cell therapy. Understanding the nature of molecular perturbations following reperfusion/reoxygenation will be highly significant for non-heart-beating donor cells. We studied non-heart-beating donor rats for global gene expression with Affymetrix microarrays, hepatic
Cristian Follmer
Phytochemistry, 69(1), 18-28 (2007-08-21)
The broad distribution of ureases in leguminous seeds, as well as the accumulation pattern of the protein during seed maturation, are suggestive of an important physiological role for this enzyme. Since the isolation and characterization of jack bean urease by
L A Winkelman et al.
Journal of dairy science, 96(12), 7565-7577 (2013-10-15)
This study investigated the effect of 2 different types of long-acting insulin on milk production, milk composition, and metabolism in lactating dairy cows. Multiparous cows (n=30) averaging 88 d in milk were assigned to one of 3 treatments in a
Gunnar Dahlén et al.
BMC oral health, 18(1), 89-89 (2018-05-20)
Urease is an enzyme produced by plaque bacteria hydrolysing urea from saliva and gingival exudate into ammonia in order to regulate the pH in the dental biofilm. The aim of this study was to assess the urease activity among oral
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What's the concentration of Jack bean Urease? I can only see the concentration of buffer.
1 answer-
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How many urease units does 1 vial contain?
1 answer-
As provided, this product contains 100 units per vial.
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What is the volume of water used for reconstitution of 1 vial?
1 answer-
As mentioned on the sample Certificate of Analysis, 30 mL of purified water in one vial of this material yields a clear solution.
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